Penetration of hamster and rabbit zona-free eggs by rat and mouse spermatozoa with special reference to sperm capacitation
نویسندگان
چکیده
منابع مشابه
Zona-free hamster ovum penetration by human spermatozoa: influence of various sperm factors.
The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by "swim-up" migration, 4-h incubation, sperm dilution to a concentration of 2.5 X 10(6) spermatozoa/ml, and observation of the oocytes 8 h after insemina...
متن کاملa comparative study of language learning strategies employmed by bilinguals and monolinguals with reference to attitudes and motivation
هدف از این تحقیق بررسی برخی عوامل ادراکی واحساسی یعنی استفاده از شیوه های یادگیری زبان ، انگیزه ها ونگرش نسبت به زبان انگلیسی در رابطه با زمینه زبانی زبان آموزان می باشد. هدف بررسی این نکته بود که آیا اختلافی چشمگیر میان زبان آموزان دو زبانه و تک زبانه در میزان استفاده از شیوه های یادگیری زبان ، انگیزه ها نگرش و سطح مهارت زبانی وجود دارد. همچنین سعی شد تا بهترین و موثرترین عوامل پیش بینی کننده ...
15 صفحه اولEffects of white blood cells on the in vitro penetration of zona-free hamster eggs by human spermatozoa.
The presence of white blood cells in semen has been associated with male infertility. Previous studies indicate that pyospermia occurs in conjunction with decreases in sperm motility, number of normal sperm forms, and penetration rates in the zona-free hamster egg sperm penetration assay. We have evaluated the relationship of seminal white blood cells and sperm function, as reflected in the zon...
متن کاملPreliminary study of buffalo sperm penetration into zona-free hamster eggs after treatment with calcium ionophore A23187.
A previous study (Takahashi & Hanada, 1984) reported the penetration of zona-free hamster eggs by ejaculated bull spermatozoa after treatment with calcium ionophore A23187 (Ca-IA) in the presence of caffeine. Based on this study, successful IVF was accomplished not only in cattle (Hanada, 1986b) but also in goats and sheep (Hanada, 1986a) using spermatozoa treated with Ca-IA. In the present stu...
متن کاملTransgenic mouse eggs with functional hamster sperm receptors in their zona pellucida.
Sperm receptors are located in the mammalian egg extracellular coat, or zona pellucida. Mouse and hamster sperm receptor glycoproteins, mZP3 (83 x 10(3) M(r)) and hZP3 (56 x 10(3) M(r)), respectively, have very similar polypeptides (44 x 10(3) M(r); 81% identical) that are glycosylated to different extents. Purified mZP3 and hZP3 can bind to mouse sperm, prevent them from binding to eggs and in...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1976
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0460239